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. 2019 Dec 10;295(7):2084–2096. doi: 10.1074/jbc.RA119.010724

Figure 2.

Figure 2.

USP7 interacts with Maf proteins. A, USP7 was identified by AP/MS/MS, and the unique peptides are highlighted in yellow. B–D, HEK293T cells were co-transfected with FLAG-USP7 and MafB (B), MafA (C), or c-Maf (D) plasmids for 48 h, and cell lysates were subjected to IP with an anti-Flag antibody and subsequent IB with indicated antibodies. E, cell lysates from MM cell lines RPMI-8226 and LP1 were incubated with an anti-MafB or USP7 antibody overnight, followed by IB with an anti-USP7 or anti-MafB antibody. F, cell lysates from MM cell lines RPMI-8226 and OCI-MY5 were incubated with an anti-USP7 antibody overnight, followed by IB with an anti-USP7 or anti-c-Maf antibody. G, The schematic draw of USP7 domains. H and I, HEK293T cells were co-transfected with Flag-USP7 truncates, HA-Ub and Myc-MafB for 44 h, followed by MG132 (20 μm) treatment for 4 h. Cell lysates were prepared for IP with an anti-Myc antibody and IB with an anti-Flag antibody.