Figure 5.

USP7 enhances Maf transcriptional activity and promotes the expression of targeted genes. A, MARE-driving luciferase plasmid was co-transfected into HEK293T cells with MafB in the presence or absence of USP7, and 24 h later, cells were collected for measurement of luciferase activity. The mutant mtMARE.Luci was used as a control. β-Gal was used as a transfection control. B, MARE-driving luciferase plasmid was co-transfected into HEK293T cells with c-Maf in the presence or absence of USP7, and 24 h later, cells were collected for measurement of luciferase activity. C, HEK293T cells were co-transfected with MafB and USP7 plasmids for 24 h, followed by cell lysate preparation and IB assays to measure the Maf target genes ITGB7 and CCND2. D, USP7 was knocked down from RPMI-8226 cells by siUSP7 (#1). Forty eight hours later, cells were subjected to IB assay against specific antibodies as indicated.