. 2020 Jan 10;295(7):1915–1925. doi: 10.1074/jbc.RA119.012078

Table 1.

Summary of kinetic parameters determined by biochemical analysis

Kinetic parameters	k_cat^a	K_m^a	k_cat/K_m	K_d^b
	s⁻¹	mg/ml	ml mg⁻¹ s⁻¹	mg/ml
WT	3.1 ± 0.21	0.32 ± 0.045	10	0.23 ± 0.019
F232W/F396W	3.9 ± 0.21	0.19 ± 0.030	21	0.18 ± 0.015

^a k_cat and K_m were estimated from the biochemical activity measurement at a low chitin concentration range (0–1 mg/ml) with the fitting by the Michaelis–Menten equation.

^b K_d was estimated from the bound fraction analysis with the fitting by Langmuir's equation.