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Enzyme assay to couple FBP/SBP formation by PFK to NADH oxidation, allowing the study of the PFK enzyme kinetics. FBA, fructose-bisphosphate aldolase; GPDH, glycerol-3-phosphate dehydrogenase; FBP, fructose 1,6-bisphosphate; G3P, glyceraldehyde 3-phosphate; Gro3P, glycerol 3-phosphate. Boxes highlight the investigated enzyme (i.e. PFK) and detected metabolite (i.e. NADH).
