Table 1.

Kinetics of the PFKs tested

Parameters of Michaelis-Menten (or Hill) kinetics were approximated by minimizing the sum of the squared vertical difference. The plots with the data points can be found in Figs. S2–S5. ATP/GTP-PFK did not show any activity with S7P, and due to the high cooperativity (n = 24) with F6P, it was not possible to fit the Michaelis-Menten equation.

			Km	kcat	n	kcat/Km
			mm	s−1		s−1 m−1
CDQ83_11320 C.ts PPi-PFK	PPi	F6P	0.070	182	NAa	2.6 × 106
		S7P	0.11	49		0.46 × 106
Clo1313_1876 C.tc PPi-PFK		F6P	0.046	127	NA	2.8 × 106
		S7P	0.10	80		0.80 × 106
CDQ83_07225 C.ts A/GTP-PFK	ATP	F6P	0.688	41	24	59 × 103
		S7P	–	–	–	–
PfkA E.co PFK		F6P	0.065	1.2	NA	19 × 103
		S7P	2.5	0.25		0.15 × 103

^a NA, not applicable. The Hill equation is only used in the case of CDQ83_07225, where n is the Hill coefficient, and K_m is replaced by K_½.