FIG 6.

Reduced accumulation of a UpA-rich RNA transcribed by the host RNA polymerase II. (A) Schematic representation of constructs transiently expressed by coagroinfiltration along with the P14 silencing suppressor. Different parts of the constructs, including the 4×MYC tag, are indicated, as well as those targeted regions in 4×MYC and NOS terminator (NOS-T) amplified for RT-qPCR analyses. (B) MYC-specific immunoblot analysis of extracts from agroinfiltrated leaf patches of N. benthamiana plants at 7 days postagroinfiltration (dpa). The membrane stained with Ponceau red that shows the RubisCO large subunit was included as a loading control. A bar graph showing the mean ± SD (n = 2) of immunoblot signals in arbitrary units is to the right (for comparison, we considered the average for F4_WT to be 1). (C) Accumulation of RNAs, estimated by RT-qPCR, corresponding to the indicated F4 fragments from agroinfiltrated leaf patches of N. benthamiana plants at 7 dpa. The result obtained for two independent pairs of primers (MYC and NOS) is depicted. Bar graphs show the mean ± SD (n = 2 plants) relative expression (for comparison, we considered the average for F4_WT to be 1).