TABLE 1.

Virulence genes upregulated in vivo

MGAS5005 gene designationa	Gene	Function	MGAS2221 gene designationb	Fold MEc	Fold ESd
Capsule
Spy1851	hasA	Hyaluronan synthase	Spy1825	11.3	31.3
Spy1852	hasB	UDP-glucose 6-dehydrogenase	Spy1826	12.0	29.2
Spy1853	hasC	UTP–glucose-1-phosphate uridylyltransferase	Spy1827	5.9	10.5
Cytolysin/exotoxin
Spy0139	nga	NAD glycohydrolase	Spy0183	5.2	35.1
Spy0140	ifs	Immunity factor for SPN	Spy0184	6.1	30.1
Spy0141	slo	Streptolysin O	Spy0185	5.7	27.8
Spy0351	spyA	c3 family ADP-ribosyltransferase	Spy0403	7.4	11.5
Spy0562	sagA	Streptolysin S precursor	Spy0598	121.0	35.1
Spy0563	sagB	Streptolysin S biosynthesis protein	Spy0599	30.8	14.1
Spy0564	sagC	Streptolysin S biosynthesis protein	Spy0600	25.6	13.8
Spy0565	sagD	Streptolysin S biosynthesis protein	Spy0601	27.3	16.9
Spy0566	sagE	Streptolysin S putative self-immunity protein	Spy0602	26.2	17.0
Spy0567	sagF	Streptolysin S biosynthesis protein	Spy0603	21.2	13.7
Spy0568	sagG	Streptolysin S export ATP-binding protein	Spy0604	25.7	16.1
Spy0569	sagH	Streptolysin S export transmembrane protein	Spy0605	32.5	19.1
Spy0570	sagI	Streptolysin S export transmembrane protein	Spy0606	27.2	16.2
Secreted/immune evasion
Spy0668	mac	IgG-degrading protease	Spy0700	4.6	8.4
Spy0981	cfa	CAMP factor	Spy0993	5.2	NSf
Spy1169	spd3	Streptodornase	Spy1179	5.3	19.4
Spy1415	sdaD2	Phage-encoded streptodornase	Spy1430	2.9	4.6
Spy1684	ska	Streptokinase	Spy0254	NS	6.3
Spy1715	scpA	C5A peptidase precursor	Spy1697	NS	3.8
Spy1718	sic	Streptococcal inhibitor of complement	Spy1699	7.6	18.5
Spy1723	ispe	Immunogenic secreted protein	Spy1703	8.4	3.5
Spy1734	spi	Streptopain protease inhibitor	Spy1713	1,080.7	NS
Spy1735	speB	Streptococcal pyrogenic exotoxin B	Spy1714	912.3	NS
Spy1738	spd	Phage-associated deoxyribonuclease	Spy1717	70.5	NS
Superantigens
Spy0182	speG	Exotoxin type G precursor	Spy0222	2.1	3.8
Spy1702	smeZ	Mitogenic exotoxin Z	Spy0238	NS	2.6
Spy0356	speJ	Exotoxin type J precursor	Spy0408	4.1	5.9
Spy0996	speA2	Phage-encoded exotoxin	Spy1009	20.4	21.8
Surface-associated/adhesins
Spy1711	lmb	Laminin binding protein	Spy0229	84.1	8.6
Spy1710	htp	Streptococcal histidine triad protein	Spy0230	80.5	8.5
Spy1687	sclA	Collagen-like surface protein A	Spy0251	NS	26.2
Spy0561	epf	Putative extracellular matrix binding protein	Spy0597	2.4	2.6
Spy0740	fbp	Fibronectin-binding protein	Spy0771	NS	2.1
Spy1109	slr	Internalin protein	Spy1122	54.1	48.6
Spy1719	emm1	M protein	Spy1700	NS	2.1
Transcriptional regulators
Spy0947	ciaH	TCSg histidine kinase	Spy0962	10.2	8.4
Spy0948	ciaR	TCS response regulator	Spy0963	14.0	12.7
Spy1318	rocA	TCS accessory protein	Spy1324	2.3	2.3
Spy1720	mga	Standalone regulator	Spy1701	1.5	2.3
Spy1724	ihk	TCS histidine kinase	Spy1704	10.5	6.5
Spy1725	irr	TCS response regulator	Spy1705	16.1	10.2
Other
Spy0281	dahA	Putative role in defense against stress	Spy0340	NS	5.0

^aGene designation for MGAS5005.

^bGene designation for MGAS2221.

^cFold differential expression comparing in vivo to in vitro ME samples.

^dFold differential expression comparing in vivo to in vitro ES samples.

^eGenes highlighted in bold were chosen for isogenic deletion-mutant strain generation.

^fNS, not significant.

^gTCS, two-component system.