Figure 3.
WIN activates an astrocytic Ca2+ signaling pathway. A–D, top, Representative GCaMP6 images from slices treated with DMSO (0.01%), WIN (3 μM), WIN (3 μM) + TTX (1 μM) + CNQX (10 μM), and WIN (3 μM) + thapsigargin (1 μM; Thap). White scale bar is 50 μm, images taken at 40× and the inserts magnified 120×. Bottom, Representative traces showing the change in fluorescence from all regions measured (gray) and average response (black) from the slice depicted above. Black arrow indicates beginning of treatment. E–H, top, Pie charts depicting percentage of soma and non-soma regions across all slices responding with an increase (black), decrease (dark gray), variable response (light gray), or no response (white) to DMSO, WIN, WIN + TTX + CNQX, or WIN + Thap (left to right). Bottom, Cumulative average fluorescence increase during baseline and drug treatment for soma and non-soma regions across all slices.