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. 2020 Feb 18;5(1):e00516-19. doi: 10.1128/mSystems.00516-19

TABLE 2.

Published experimental data used during the validation process

Phenotype or metabolic characteristics Reference(s)
Reduction of nitrogen to ammonia by nitrogenase reaction 15
Malate as carbon source 86
Succinate as carbon source 86
Alanine production by bacteroid 64
Production of PHB 51
pyrC mutant: Fix 42
pyrF mutant: Fix 48
C4-dicarboxylate requirement 62
purL mutant: Fix 47, 49
purQ mutant: Fix 47
idhA mutant: decrease nitrogen fixation 66
Glutamate requirement: enhances N2 fixation 38
znuA mutant: reduce nitrogen fixation 17
ptsSCAB mutant: reduce nitrogen fixation 52
cobO mutant: reduce number of nodules 43