Figure 2.

Chromosome Transplantation in Human LN-iPSCs

(A) Representative karyotype (left) and metaphase spread (right) after FISH with a probe (green) for the human X chromosome of the HAT-resistant clones (HAT^R) after retro-MMCT. Chromosomes were counterstained with DAPI (blue). (B) PCR analysis of the HPRT locus in four CT-corrected clones (LNX-D1B, LNX-N9.5, LNX11.2, and LNX12.3) in the HPRT mutated LN-iPSCs and in the HPRT WT control cells, respectively. Neg, negative control. 1 kb, DNA ladder. The 461-bp band, specific for the exon1, is seen only in the HPRT WT control and in the CT clones. (C and D) Analysis of LNX-N11.2 (C) and LNX-N12.3 (D) clones. Left, histogram showing the chromosome distribution; middle, representative DAPI-banding karyotype; and right, metaphase spread after FISH with a human X chromosome painting probe (red). Chromosomes were counterstained with DAPI (blue). p, passage number.