Figure 3.

Cytogenetic Characterization of the HAT^R LNX-D1 Clone after the Second Round of Reprogramming

(A and B) Analysis of the LNX-D1 clone at p4 (A) and p10 (B). Left, histograms showing the karyotype distribution; and right, representative metaphase spreads after FISH using a human X chromosome painting probe (red). Loss of one of the X chromosomes occurred after few passages in culture (p10). p, passage number. (C) Analysis of the CT 46,XY LNX-D1B subclone. Left, histogram showing the chromosome distribution; middle, representative DAPI-banding karyotype; and right: metaphase spread after FISH with a human X chromosome painting probe (red). Chromosomes were counterstained with DAPI (blue). p, passage number.