Table 3. The fold changes of the fermentation parameters of wild-type (WT) vs its mutant atg32△ sake yeast cells cultured in minimal synthetic medium containing 15% glucose after the addition of amino acids (AA).
| Sample no. | γ(CO2)/(g/L) | Max. fermentation rate/ (g/(L·day)) | φ(ethanol)/% | Growth profile (A600 nm. 107 cells/mL) |
||||
|---|---|---|---|---|---|---|---|---|
| Fold change (WT/atg32Δ) | p-value | Fold change (WT/atg32Δ) | p-value | Fold change (WT/atg32Δ) | p-value | Fold change (WT/atg32Δ) | p-value | |
| WT/atg32△(Met) | 0.43/-1.04 | 0.011 | 1.04/0.36 | 0.021 | 0.07/-0.06 | 0.022 | -0.12/0.06 | 0.006 |
| WT/atg32△(Cys) | 4.43/1.90 | 0.001 | 1.20/0.53 | 0.029 | 0.17/0.07 | 0.039 | -0.12/0.08 | 0.004 |
| WT/atg32△(Gly) | 0.77/-0.07 | 0.055 | 0.97/0.40 | 0.045 | 0.17/0.07 | 0.008 | -0.08/0.09 | 0.022 |
| WT/atg32△(Ala) | 3.00/1.76 | 0.065 | 0.94/0.20 | 0.010 | 0.14/0.06 | 0.095 | 0.03/0.13 | 0.175 |
| WT/atg32△(Val) | 4.00/2.40 | 0.062 | 1.20/0.43 | 0.024 | 0.19/0.09 | 0.080 | -0.06/0.07 | 0.049 |
| WT/atg32△(Pro) | 2.90/-0.50 | 0.001 | 1.20/0.36 | 0.007 | 0.15/0.02 | 0.009 | -0.12/0.08 | 0.005 |
| WT/atg32△(Phe) | 1.60/-2.57 | 0.001 | 0.60/0.20 | 0.153 | 0.02/0.13 | 0.067 | 0.10/0.15 | 0.521 |
| WT/atg32△(Leu) | 3.10/-0.07 | 0.001 | 0.94/0.59 | 0.256 | 0.15/0.01 | 0.021 | 0.01/0.10 | 0.132 |
| WT/atg32△(Ile) | 1.56/-2.64 | 0.001 | 0.47/-0.14 | 0.051 | 0.03/0.16 | 0.133 | 0.05/0.15 | 0.131 |
| WT/atg32△(Tyr) | 2.83/-0.27 | 0.001 | 0.97/0.33 | 0.027 | 0.17/0.02 | 0.006 | -0.13/0.08 | 0.003 |
| WT/atg32△(Trp) | 2.13/-1.50 | 0.001 | 0.74/0.36 | 0.196 | 0.10/-0.01 | 0.021 | 0.06/0.13 | 0.319 |
| WT/atg32△(Ser) | 3.07/-0.03 | 0.001 | 1.07/0.36 | 0.007 | 0.15/0.04 | 0.037 | -0.09/0.08 | 0.016 |
| WT/atg32△(Thr) | 1.97/1.90 | 0.886 | 1.12/0.66 | 0.118 | 0.17/0.14 | 0.499 | 0.02/0.06 | 0.582 |
| WT/atg32△(Gln) | 1.93/-0.44 | 0.001 | 0.97/0.46 | 0.062 | 0.15/0.02 | 0.035 | -0.01/0.10 | 0.052 |
| WT/atg32△(Lys) | 0.87/1.44 | 0.295 | 0.50/-0.04 | 0.040 | 0.05/-0.06 | 0.028 | 0.03/0.12 | 0.210 |
| WT/atg32△(Arg) | 3.20/1.53 | 0.061 | 0.94/0.43 | 0.095 | 0.20/0.06 | 0.011 | -0.05/0.08 | 0.055 |
| WT/atg32△(His) | 3.17/0.56 | 0.001 | 0.90/0.43 | 0.062 | 0.12/0.02 | 0.047 | 0.02/0.09 | 0.269 |
| WT/atg32△(Asp) | 3.30/0.86 | 0.001 | 1.17/0.73 | 0.176 | 0.17/0.12 | 0.255 | -0.04/0.07 | 0.052 |
| WT/atg32△(Glu) | 2.03/1.53 | 0.345 | 0.97/0.46 | 0.062 | 0.15/0.06 | 0.053 | 0.05/0.12 | 0.201 |
| WT/atg32△(Asn) | 1.80/1.73 | 0.892 | 0.90/0.60 | 0.282 | 0.13/0.07 | 0.080 | 0.05/0.08 | 0.736 |
The statistical significance of interaction was analyzed using two-way factorial ANOVA (p<0.05). For amino acid abbreviations see Table 1