Table 1.
PCR primers, accession numbers, amplicon sizes, and PCR efficiencies.
| Gene symbol | Gene name | Potential marker for | Accession no. | Forward primer | Reverse primer | Amplicon size (bp) | PCR efficiency |
|---|---|---|---|---|---|---|---|
| dnmt1 | DNA (cytosine-5-)- methyltransferase 1 |
DNA methylation | NM_131189 | GGGCTACCAGTGCACCTTTG | GATGATAGCTCTGCGTCGAGTC | 76 | 1.91 |
| dnmt3aa | DNA (cytosine-5-)- methyltransferase 3A |
DNA methylation | NM_001018134 | GGCGCCTGTTCTTTGAGTTT | TCACTGACCCCCATTGCAA | 112 | 1.91 |
| dnmt3b | DNA (cytosine-5-)- methyltransferase 3B |
DNA methylation | NM_001020476 | AGGTTTGGAACCTCCCGAAA | TGCGCACAGGTAACAAATGG | 115 | 1.94 |
| cbs | Cystathionine-beta-synthase | Transsulfuration | NM_001111232 | CTTTGCCCTGGTGGTTCATG | ACCACTCCAAACACCATTTGC | 81 | 2.00 |
| mgmt | O-6-methylguanine-DNA methyltransferase | DNA repair | NM_001256243 | TCCACCCTGTTGTCCTGTCA | GATGTAAGGCAGGCAGAGGAA | 117 | 2.03 |
| pgrmc1 | Progesterone receptor membrane component 1 | Glucose/Energy metabolism | NM_001007392 | TTTTCACGTCGCCACTGAAC | CTCCTCAACCGGGCCATAGT | 104 | 1.90 |
| cyp1a1 | Cytochrome P450 family 1 subfamily A member 1 | Detoxification | AF210727 | GGTGTTGGTTTTCGGTTTGG | GGCATCCCGGTGAACTTTAA | 114 | 1.99 |
| vtg1 | Vitellogenin 1 | Endocrine disruption | NM_001044897 | GTCATCAATGAGGATCCAAAGGCCA | GCCTCAGCGATCAGTGCACCAT | 209 | 1.91 |
| esr1* | Estrogen receptor 1 | Endocrine disruption | NM_152959 | AAACACAGCCGGCCCTACAC | GCCAAGAGCTCTCCAACAAC | 157 | 2.12 |
| esr2a* | Estrogen receptor 2a | Endocrine disruption | NM_180966 | TGATCAGCTGGGCCAAGAAG | GATTAACGGAGCGCCACATC | 123 | 2.00** |
| ar | Androgen receptor | Endocrine disruption | NM_001083123 | GGATGAGGTCGGAGCAGTTC | GGCTCAATGGCCTCCAGAAT | 117 | 2.03 |
| cyp19a2 | Cytochrome P450 family 19 subfamily A member 2 | Endocrine disruption | AF406756 | GAGCGGGCAGGACATAGTGT | GCTTGGGCTCAATCACTCTCA | 89 | 2.10 |
| fos | Fos proto-oncogene | Cell proliferation, differentiation and transcription regulation | NM_205569 | GGGTATTACCCGCTCAACCA | CAAGTCCGGGCATGAAGAGA | 102 | 2.02 |
| mapk1 | Mitogen-activated protein kinase 1 | Cell proliferation, differentiation and survival | NM_182888 | TACATCGGAGGAGGCGCTTA | GCTCAAACGGGCTGATCTTC | 94 | 1.99 |
| casp3a | Caspase 3A | Apoptosis | NM_131877 | CCCAGATGGTCGTGAAAGGAT | TGAACCATGAGCCGGTCATT | 107 | 2.07 |
| eef1a1 | Eukaryotic translation elongation factor 1 alpha 1 | Refgen | AY422992 | AGACAACCCCAAGGCTCTCA | CTCATGTCACGCACAGCAAA | 126 | 2.06 |
| uba52 | Ubiquitin A-52 residue ribosomal protein fusion product 1 | Refgen | NM_001037113 | CGAGCCTTCTCTCCGTCAGT | TTGTTGGTGTGTCCGCACTT | 126 | 2.08 |
| actb | Beta-actin | Refgen | AF057040 | CGAGCAGGAGATGGGAACC | CAACGGAAACGCTCATTGC | 102 | 2.08 |
*PCR primers obtained from Sawyer et al. [93]. **PCR efficiency set to 2.00.