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. 2020 Feb 19;19:42. doi: 10.1186/s12934-020-01308-1

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — TTFC adsorption on B. subtilis spores. a Western blotting of spore surface proteins after adsorption with 2.0 µg of purified TTFC. Lanes 1: purified TTFC; 2: proteins extracted from adsorbed spores; 3: proteins extracted from adsorbed spores after 1 week storage at 4 °C; 4: five-fold concentrated supernatant after 1 week storage at 4 °C. b Dot blotting experiment performed with the serial dilutions of the supernatant (unbound TTFC) fraction of the adsorption reaction. Serial dilutions of purified TTFC were used as a standard. c Flow cytometry analysis of: free spores incubated (brown histogram) or not (red histogram) with specific antibodies and TTFC-adsorbed spores incubated with specific antibodies (filled blue histogram). The analysis was performed on the entire spore population (ungated). Immune-reactions were performed with polyclonal anti-TTFC [17] and anti-rabbit HRP conjugate (panels A and B) or with FITC-conjugated secondary antibodies (panel C)