Fig. 2.
Experimental plan. Three experimental groups received the oral probiotic treatment (yellow lines) with 1.0 × 106 spores/gram from day -7 to day 35. One of these groups was immunized with 2.0 μg of purified TTFC (blue arrows) and another one with 2.0 × 109 spores adsorbed with TTFC (Sp-TTFC) (red arrows) on day 0, 14 and 28. All immunizations were performed by the nasal route. Two groups were immunized only with purified TTFC or Sp-TTFC without probiotics. A naive group that did not receive either probiotics or the antigen was also included. Blood samples were collected from all animals at days 0, 14 and 21 and 35, at day 35 all animals were sacrificed for analysis