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. 2020 Feb 19;19:42. doi: 10.1186/s12934-020-01308-1

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — IgG subclasses analysis. The phenotype of the induced humoral immune response. Anti-TTFC IgG1 (a), IgG2b (b), IgG2c (c), and IgG3 (d) levels detected in mice serum on days 14, 21, and 35. Not-immunized (naïve and probiotic) groups did not produce anti-TTFC antibodies and were not reported in the figure. The data represent the mean (± standard error) of reciprocal endpoint titers. Equal letters mean no statistical difference (p > 0.05) and different letters mean a statistical difference (p < 0.05) between the experimental groups