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Densitometric analysis of dot blot experiments of Fig. 1b with the supernatants of the adsorption reaction with wild type spores
| TTFC source | Amount of sample used | Density (OD/mm2) | Amount of TTFC (ng) |
Amount of TTFC μg in 200 μl (% total) |
|---|---|---|---|---|
| Purified TTFC | 50.00 ng | 29.053 | NA | NA |
| 25.00 ng | 13.121 | NA | NA | |
| 12.50 ng | 5.294 | NA | NA | |
| Free TTFC | 80 μl | 16.505 | 23.4 | 0.05 (2.9) |
| (supernatant) | 40 μl | 8.012 | 11.8 | 0.05 (2.9) |
| 20 μl | 4.629 | 4.5 | 0.04 (2.2) |
