Fig. 3.

Differentiation of SBMA disease specific iPSCs into motor neurons. a Schematic presentation of the culture protocol for the differentiation of iPSCs. LSC, LDN-193189 (L), SB4315342 (S), CHIR99021 (C); RA, retinoic acid; PM, purmorphamine. b Immunocytochemical analysis of HB9, ISL-1, and βIII-Tubulin after 1 week of monolayer differentiation with 10 nM DHT. Scale bar, 100 μm. c Quantitative RT-PCR analysis of HB9, ISL-1, ChAT, and AR expressions at 4 weeks (n = 4, mean ± SEM). HB9 and ISL-1 expressions were higher in SBMA than controls in the presence of 10 nM DHT (*p < 0.05; Student’s t-test)