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. 2020 Feb 19;13:18. doi: 10.1186/s13041-020-0561-1

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© The Author(s). 2020

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Purification of HB9^e438::Venus positive MNs by flow cytometry. a Schematic presentation of the culture protocol for flow cytometry and cell sorting. b Schematic histogram for determining a negative fraction (NF) and high-positive fraction (HPF). NF consists of uninfected cells (gray). HPF consists of top 2/3 fraction of HB9^e438::Venus⁺ cells (green). NT consists of unpurified cells. Blue, background lentiviral infected cells. c Quantitative RT-PCR analysis of HB9, ISL-1, ChAT, AR, and GFAP expressions at 4 weeks. Data are normalized to β-ACTIN and presented as the relative expressions to controls (n = 4, means ± SEM). Significant increases in the expressions of HB9, ISL-1, and ChAT are observed in the HPF. A significant decrease in the expression of GFAP is observed in the HPF (*p < 0.05; Student’s t-test)