Fig. 2.

PilG and PilH influence diverse cellular processes including chemotaxis and virulence.a Chemotaxis response assay. The strains were diluted and performed simplified capillary assays for 8 chemoattractant and counted bacterial CFUs then incubating at 28 °C for 3 days. Relative chemotaxis fraction was calculated CFU in test capillary vs CFU in control buffer capillary. b The virulence of Xcc strains. The strains were diluted and sprayed onto leaves of 100 radishes showing similar growth, and counted diseased leaves rate after 10 days. Additionally, each strain was repeated three times. c In-plant growth curve. The strains were diluted and inoculated to Chinese radish by leaf-clipping. At intervals of 1 day, four clipped leaves for every group of inoculated plants were collected and homogenized, and then diluted to plate on NYG plates. The colonies were counted after 3 days of incubation at 28 °C. Significance was tested by Student’s t test (* and ** represent significance at P < 0.05 and 0.01, respectively)