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. 2020 Feb 18;20:37. doi: 10.1186/s12866-020-1712-3

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Phenotypic characterization of mutation in six motility-related (pili, flagellar and chemotaxis) genes. a Examination of swimming and swarming motility. Left side, strains were inoculated onto ‘swarm’ plate (NY medium containing 2% glucose and 0.6% agar) then incubated at 28 °C for 3 days. Right side, strains were stabbed into ‘swim’ plate (0.03% Bacto peptone, 0.03% yeast extract and 0.28% agar) then incubated at 28 °C for 3 days. b The diameter of the strains on swimming and swarming plates. c Chemotaxis response assay. The strains were diluted and performed simplified capillary assays for 8 chemoattractant, and counted bacterial CFUs after 3 days of incubation at 28 °C. Relative chemotaxis fraction was calculated CFU in test capillary vs CFU in control buffer capillary. All datas were representative of three independent experiments. Significance was tested by Student’s t test (* and ** represent significance at P < 0.05 and 0.01, respectively)