Table 2.

In vitro activities of some key enzymes in genetically modified C. glutamicum strains and original strain C. glutamicum K-1 on CgXII^IP-medium with glucose as carbon source

Strains	PTSGlc	GlK		PFK		ScrK	PPC	PYK	NDH-2	FoF1-ATPase
		ATP	ADP	ATP	ADP
K-1	1.73 ± 0.27	0.046 ± 0.004	ND	0.13 ± 0.01	ND	ND	0.50 ± 0.09	1.47 ± 0.10	7.31 ± 1.52	0.39 ± 0.04
K-2	1.71 ± 0.12	0.045 ± 0.001	–	0.15 ± 0.02	–	0.11 ± 0.02	0.51 ± 0.12	1.47 ± 0.18	8.16 ± 2.03	0.44 ± 0.02
K-3	≤ 0.1	0.057 ± 0.003	–	0.02 ± 0.00	–	0.03 ± 0.02	0.17 ± 0.05	0.56 ± 0.09	2.35 ± 0.55	0.13 ± 0.02
K-4	–	0.073 ± 0.007	–	0.07 ± 0.02	–	0.07 ± 0.05	0.42 ± 0.05	1.14 ± 0.15	7.88 ± 1.64	0.41 ± 0.03
K-5	–	0.312 ± 0.018	–	0.21 ± 0.04	–	0.09 ± 0.02	0.95 ± 0.14	2.23 ± 0.21	8.43 ± 1.78	0.52 ± 0.07
K-6	–	0.325 ± 0.021	0.21 ± 0.01	0.26 ± 0.03	0.15 ± 0.03	0.11 ± 0.06	0.79 ± 0.07	2.78 ± 0.18	8.45 ± 2.25	0.53 ± 0.01
K-7	–	0.331 ± 0.019	0.21 ± 0.03	0.27 ± 0.05	0.14 ± 0.03	0.11 ± 0.03	0.80 ± 0.13	2.74 ± 0.25	26.27 ± 8.76	0.60 ± 0.08
K-8	–	0.354 ± 0.043	0.18 ± 0.04	0.32 ± 0.04	0.10 ± 0.02	0.13 ± 0.05	0.87 ± 0.09	2.59 ± 0.16	29.73 ± 5.84	0.91 ± 0.05

The unit of specific enzyme activity is U/(mg protein)

All data are meaning values of three determinations of three independent experiments with ± SD

“–” represents no test, and ND represents no detection