Table 5.
Strains used in this study
| Contents | Relevant characteristic(s) | References |
|---|---|---|
| C. glutamicum strains | ||
| K-1 | C. glutamicum JL-6 ∆pck::ppc ∆odx::pyc ∆P1gltA/Ptac-Mgdh, l-lysine-producing strain derived from strain C. glutamicum ATCC13032 | [3] |
| K-2 | Derivative of strain K-1 with hetero-expression of scrK from C. acetobutylicum | This work |
| K-3 | Derivative of strain K-2 with deletion of ptsG and ptsF | This work |
| K-4 | Derivative of strain K-3 with two point mutations in the promoter of iolT1, relative to the start codon at position-113 (A → G) and -112 (C → G) respectively, and with replacement of natural promoter of gene iolT2 by tuf promoter as well as two copies of iolT2 gene | This work |
| K-5 | Derivative of strain K-4 with replacement of natural gene glkCg by the optimized gene glkBs from B. subtilis | This work |
| K-6 | Derivative of strain K-5 with introduction of the optimized gene glkMm from M. maripaludis | This work |
| K-7 | Derivative of strain K-6 with replacement of natural promoter of gene ndh by tuf promoter | This work |
| K-8 | Derivative of strain K-7 with deletion of the gene sigH | This work |
| Plasmids | ||
| pK18mobsacB | Integration vector | [59] |
| pK18mobsacB/∆pfkB::scrKOp.Ca | Integration vector for introduction of the cassette of scrK gene at pfkB gene loci | This work |
| pK18mobsacB/∆ptsG | Integration vector for deletion of ptsG gene | This work |
| pK18mobsacB/∆ptsF | Integration vector for deletion of ptsF gene | This work |
| pK18mobsacB/∆ptsF::glkOp.Mm | Integration vector for introduction of the cassette of glkMm gene at ptsF gene loci | This work |
| pK18mobsacB/∆glkCg::glkOp.Bs | Integration vector for replacement of the native glkCg gene by the cassette of glkBs gene | This work |
| pK18mobsacB/PO6iolT1 | Integration vector for introducing two point mutations in the promoter region of iolT1, relative to the start codon at position-113 (A → G) and -112 (C → G), respectively | This work |
| pK18mobsacB-PtufiolT2 | Integration vector for replacement of the nature promoter of iolT2 gene by the tuf promoter | This work |
| pK18mobsacB-2 × iolT2 | Integration vector for introducing a second copy of the iolT2 gene with tuf promoter | This work |
| pK18mobsacB-Ptufndh | Integration vector for replacement of the nature promoter of ndh gene by the tuf promoter | This work |
| pK18mobsacB/∆sigH | Integration vector for deletion of sigH gene | This work |