Table 2.

Primer sequences and characteristics of 12 polymorphic microsatellite loci

Locus	Chromosome arm	Repeat motif	No. of alleles	Allele size	Primer sequence (5′-3′)	Reference
FunL F	2L	(GT)8	12	181–197	HEX-AACAGTGGAAGGCAAATTGC	Cohuet et al. [22]
FunL R					GCACGGTTACCACTGCTCA
AFUB10 F	2L	(GCT)3+2+4+5	6	195–210	PET-TGTCCATGTACAACCGCAAC	Sharakov et al. [23]
AFUB10 R					TTCTCCAGCATCATCAGCAC
AFUB11 F	2L	(CTG)3+5+2+2	4	188–191	PET-CAGTTTCTGCGTGGAGGAAT	Sharakov et al. [23]
AFUB11 R					AGCAGCTGATGAGCCATCTC
AFND23 F	2L	(GT)11	11	133–157	6-FAM-TTTGATCGACGGACTAGTGTGT	Schemerhorn et al. [25]
AFND23 R					GGTTTGATGGGTGGAAAC
FunO F	2R	(CA)6T(AC)4	10	110–132	PETGCACACATTTCAGGCAGC	Cohuet et al. [22]
FunO R					GCCCACATTCTGCACCTT
AFUB3 F	2R	(CAG)2+3+2	5	171–195	VIC- GGGAAGGATTCGACCTTAGC	Sharakov et al. [23]
AFUB3 R					GCCGCCATTTAGTAGCAGTT
FunF F	3L	(TG)9	7	104–118	6-FAM –GCCTTCAGTTTCGATTGGCG	Cohuet et al. [22]
FunF R					AATAAGATGCGACCGTGGC
AFUB12 F	3L	(AGG)7(TG)4	3	152–158	VIC –TGGGGAACTGGTCGTTAGAG	Sharakov et al. [23]
AFUB12 R					CTGGTGATGGGATTGAGGAT
AFND19 F	3R	(AG)12(TG)5	8	251–285	HEX –GCAAGCTGTACGCAGAGAG	Sharakov et al. [24]
AFND19 R					ATCGATGGGAGTTATTATACGC
AFND20 F	3R	(GAG)4+2+2+2(TGG)3	2	239–242	VIC- CGGCGCAGGTTTAGTAGC	Sharakov et al. [24]
AFND20 R					CCCTCGCTTTCCTCATAAAA
AFND41 F	3R	(CA)3TC(CA)6	6	222–246	6-FAM AGAACATATGGCAAATCGAC	Schemerhorn et al. [25]
AFND41 R					GAAAGACTTGTCGGACGTG
FunQ F	X	(TG)9	7	84–98	HEX –GCAAACTGCTAGTAAATGTTTCC	Cohuet et al. [22]
FunQ R					ACATTTCCACAATTTGCGC