Fig. 5.

Preventive ADAMTS13 treatment reduced VWF levels in spinal cords and preserved blood-spinal cord barriers (BSCBs) of EAE mice. a Tissue sections of spinal sections from EAE mice at 22 dpi were stained with antibodies against VWF (red) and DAPI (blue, nucleus). Bars, 20 μm. b Quantitative RT-PCR analysis of VWF mRNA levels in spinal cords of EAE mice at 22 dpi. Data represent fold change compared to the spinal cords of naïve mice. c Mice received intravenous an Evans blue injection at 22 dpi, and the amount of Evans blue was averaged by the weights of spinal cord tissue. Values are expressed as the mean ± SEM (n = 5–6 each group). Student’s t test (b) and one-way ANOVA followed by Dunnett’s posttest (c) were used to compare differences. **P < 0.01; ***P < 0.001