Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Feb 18;21:56. doi: 10.1186/s12931-020-1319-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 1 — IPF-HLFs secrete high levels of IL-6 and activate the STAT3 pathway in normal HLFs. Human lung fibroblasts from patients with IPF (IPF-HLF) or control donors (N-HLF) and were cultured and their supernatants (SN) were collected. IL-6 levels in the SN were measured by ELISA-based array a. IL-6 mRNA levels from N-HLF and IPF-HLF cells were measured by qPCR b SN from IPF-HLFs was added to N-HLF for further cultures. The effect of the IPF-HLF-SN on N-HLF pSTAT3-Y705 (30 min, c-d, pSTAT3-S727 (24 h, e and total protein levels of SOCS3 (24 h, f were analyzed by western blotting. c Representative western blots for Figs. D-F. *p < 0.05, **p < 0.01, and ***p < 0.001. (n ≥ 4).