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. 2020 Feb 18;21:56. doi: 10.1186/s12931-020-1319-0

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Phosphorylated and total Smad3 are overexpressed in IPF-HLFs. Protein interaction networks were constructed using STRING (http://string-db.org/) a. Proteins and RNA were extracted from human lung fibroblasts from patients with IPF (IPF-HLF) or control donors (N-HLF). phospho and total Smad3 protein levels were tested using Western Blot b-c. Smad3 d and soluble IL-6R e mRNA levels were measured by qPCR. *p < 0.05, **p < 0.01, and ***p < 0.001 (n ≥ 4)