Table 1.
List of proteins identified by GeLC-MS/MS analysis of A. foliaceus secretory/excretory products showing hits with OmicsBOx (Blast2Go) database
| Family/Domain | SeqName | Description | MW (kDa) | No. Peptides | SC (%) | Pro. length | E-value | SignalP | Hits Organism | Function | NCBI ID |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Vitellogenin_N | afol_3429.1p | Von Willebrand factor type D domain protein | 172.5 | 55 | 39.4 | 1499 | 8.39E−11 | Yes | Ixodes scapularis | Transporters, osmolality, immunity and clotting [25, 51–53] | XP_029822532.1 |
| afol_51180.1p | Vitellogenin-like isoform X1 | 183.8 | 44 | 30.1 | 1582 | 1.26E−11 | No | Ixodes scapularis | XP_029826448.1 | ||
| Hemocyanin | afol_43548.1p | Hemocyanin subunit type 1 precursor | 81.2 | 18 | 26.3 | 697 | 0 | Yes | Argulus foliaceus | Respiratory, protein storage [54] | CUH82791.1 |
| afol_52001.1p | Hemocyanin A chain | 36.2 | 8 | 30.6 | 314 | 0 | No | Argulus foliaceus | CUH82792.1 | ||
| Astacin | afol_16671.1p | Protein SpAN-like | 72.6 | 11 | 21.2 | 660 | 1.49E−55 | Yes | Saccoglossus kowalevskii | Immunity; antifungal activity [55], food digestion, host penetration, immune evasion or activation [56–58] | XP_002739691.1 |
| afol_52344.2p | Protein SpAN-like | 76.4 | 12 | 17.6 | 687 | 2.85E−50 | No | Penaeus vannamei | XP_027221531.1 | ||
| afol_18725.8p | Blastula protease 10-like | 50.7 | 8 | 18.4 | 462 | 1.44E−17 | No | Penaeus vannamei | XP_027219989.1 | ||
| Serpin | afol_27409.2p | Serpin B6-like | 51.4 | 7 | 20.0 | 461 | 6.15E−66 | No | Rhipicephalus microplus | Anticoagulation activity, modulate host immune response, regulation of host inflammation, antihaemostatic effects, and platelet aggregation [59–64] | AHC98669.1 |
| afol_25414.2p | Leukocyte elastase inhibitor-like | 11.2 | 4 | 35.1 | 94 | 1.05E−16 | No | Ixodes scapularis | EEC05896.1 | ||
| Fasciclin | afol_20127.1p | Beta-ig-h3 fasciclin | 37.1 | 5 | 23.1 | 333 | 1.64E−22 | Yes | Culex quinquefasciatus | Mediate cell adhesion [65, 66] | XP_001847648.1 |
| Trypsin | afol_3246.7p | Transmembrane protease serine 9 | 28.1 | 5 | 21.4 | 262 | 2.81E−12 | No | Penaeus vannamei | Digestion, maintain host-parasite relationship [67] | XP_027224040.1 |
| afol_16878.20p | Trypsin-1 | 32.0 | 9 | 34.5 | 293 | 6.81E−61 | Yes | Ceratitis capitata | XP_004520346.1 | ||
| afol_19181.1p | Serine protease 29 | 47.0 | 7 | 24.5 | 466 | 8.72E−87 | Yes | Bombus terrestris | XP_020722765.1 | ||
| afol_18345.1p | Transmembrane protease serine 9-like | 18.3 | 4 | 25.6 | 164 | 1.38E−17 | No | Drosophila navojoa | XP_030246706.1 | ||
| afol_51127.2p | Trypsin | 25.1 | 3 | 15.7 | 236 | 2.13E−57 | No | Penaeus vannamei | ROT79324.1 | ||
| afol_56688.3p | Serine protease1/2 | 29.1 | 2 | 6.1 | 262 | 3.42E−39 | Yes | Ixodes scapularis | XP_029851465.1 | ||
| Peptidase_M14 | afol_12392.3p | Mast cell carboxypeptidase A | 48.3 | 5 | 16.7 | 424 | 4.21E−88 | No | Penaeus vannamei | Proteolytic-enzyme [68]) | XP_027226524.1 |
| VIT | afol_32337.1p | Inter-alpha-trypsin inhibitor heavy chain H4-like isoform X2 | 98.2 | 4 | 7.4 | 883 | 2.31E−151 | Yes | Lingula anatina | Proteinase inhibitor [36] | XP_013407760.1 |
Note: Description of suggested functions reported in different species of arthropods, and were blasted against Metazoa in NCBI BLASTP to obtain an Accession No. Signal P was used to predict the secretory property of the protein
Abbreviations: MW, molecular weight of protein; SC, sequence coverage; E-value, from the OmicsBox output