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. 2019 Dec 21;7(4):1900949. doi: 10.1002/advs.201900949

Figure 6.

Figure 6

Circ‐MALAT1 absorbs miR‐6887‐3p to upregulate JAK2 in vivo. A) After treatment with miR‐6887‐3p mimic, miR‐512‐5p mimic, or its scrambled version (control mimic), both circ‐MALAT1 and JAK2 mRNA of xenograft tumors were detected by qRT‐PCR (top panel), and JAK2 expression at the protein level was detected by western blot (bottom panel). miR‐512‐5p was used as a control miRNA. GAPDH was used as a control primer. B) After treatment with miR‐6887‐3p inhibitor, miR‐512‐5p inhibitor or its scrambled version (control inhibitor), both circ‐MALAT1 and JAK2 mRNA of xenograft tumors were detected by qRT‐PCR (top panel), and JAK2 expression at the protein level was detected by western blot (bottom panel). miR‐512‐5p was used as a control miRNA. GAPDH was used as a control primer. C) MiR‐6887‐3p expression was analyzed in the xenograft tumors generated by circ‐MALAT1 overexpressed (circ‐MALAT1 O/E) or empty vector (Vector) Huh7 cells. Control primers, GAPDH and U6. D) p‐JAK2 was determined in the xenograft tumors generated by circ‐MALAT1 overexpressed (circ‐MALAT1 O/E) or empty vector (Vector) Huh7 cells. Columns, means from three independent experiments; bars, SD. * p < 0.05.