Figure 4.

RNA-seq analyses based on gene metrics, like Phylo-DE, enable analysis of all measured mRNAs. (A) Genes lacking GO term annotations for commonly studied yeast species. (B) GO annotations are shown from Ensembl biomart using current GO terms (2019). (C) Examples of RNA-seq data analysis using attributes like GO Terms (gray) compared to analyses using metrics like gene age (beige). (D) The phylostrigraphy methodology is shown, gene age is inferred by the identification of an orthologous gene in organisms with decreasing last common ancestor evolutionary distance. (E) Hypothetical Phylo-DE analysis showing no relationship between gene age and DE. (F) Hypothetical Phylo-DE analysis showing enrichment for young gene DE.