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. 2019 Apr 30;30(1):256–268. doi: 10.1093/cercor/bhz085

Figure 6.

Figure 6

Complexity of PNNs is increased in Mecp2 KO organotypic cultures. (A) Double immunostaining of PNNs (red) and NeuN (blue) in cortical organotypic cultures of Mecp2 WT and KO mice. Scale bar: 20 μm. (B, C) Quantification of the number of WFA-positive cells reveals an increase in the density of PNN-positive enwrapped neurons (B) and in the WFA-positive dendritic length (C) in Mecp2 KO culture. Mann–Whitney test, *P ˂ 0.05; ***P ˂ 0.001. (D, E) Percentage of both WFA-positive primary (D) and secondary neurites (E) is increased in Mecp2 KO cultures as compared to WT cultures. n = 5 animals per genotype. Two-way ANOVA, ***P < 0.001, Bonferroni’s post-test. Mean ± s.e.m.