Table 1.
Adult dysregulation of inhibitory system in visual cortex of Mecp2 KO mice
| P30 | P60 | |||
|---|---|---|---|---|
| Gene | Mecp2 WT | Mecp2 KO | Mecp2 WT | Mecp2 KO |
| Gad2 | 1.1 ± 0.04 | 1.1 ± 0.03 | 0.9 ± 0.02## | 0.8 ± 0.02$$$** |
| Gad1 | 1.1 ± 0.1 | 1.1 ± 0.03 | 1.0 ± 0.04 | 0.9 ± 0.03$$$ |
| Kcnc1 | 1.1 ± 0.1 | 1.2 ± 0.1 | 1.0 ± 0.02 | 0.9 ± 0.03$ |
| Slc32a1 | 1.0 ± 0.1 | 1.2 ± 0.03 | 1.1 ± 0.03 | 1.0 ± 0.01$$ |
| Gphn | 0.9 ± 0.1 | 1.3 ± 0.1 | 1.0 ± 0.04 | 1.0 ± 0.04 |
| Gabra1 | 1.0 ± 0.1 | 0.1 ± 0.03 | 0.9 ± 0.04 | 0.9 ± 0.01 |
| Gabra2 | 1.8 ± 0.1 | 1.0 ± 0.1 | 1.0 ± 0.02 | 0.9 ± 0.03 |
| Pvalb | 1.0 ± 0.05 | 1.1 ± 0.03 | 0.9 ± 0.03 | 1.1 ± 0.03** |
| Calb1 | 1.5 ± 0.2 | 1.4 ± 0.1 | 0.9 ± 0.1### | 0.7 ± 0.03$$$ |
| Calb2 | 1.5 ± 0.3 | 1.5 ± 0.1 | 0.9 ± 0.03## | 0.7 ± 0.03$$$ |
| Sst | 1.2 ± 0.1 | 1.2 ± 0.1 | 1.2 ± 0.1 | 0.9 ± 0.1* |
Quantitative mRNA expression level of visual cortical homogenates demonstrated no differences between Mecp2 WT and Mecp2 KO at P30. At P60, a general downregulation of some of the inhibitory markers. Only parvalbumin (Pvalb) mRNA levels were upregulated. P30, WT, KO n = 3; P60, WT, KO n = 4–6 mice. One-way ANOVA, *P < 0.05; **P < 0.01, Bonferroni post-test. # indicates significance between P30 and P60 WT, $ indicates significance between P30 and P60 KO and * indicates significance between P60 WT and P60 KO. Mean ± s.e.m.