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. Author manuscript; available in PMC: 2021 Jan 31.
Published in final edited form as: Circ Res. 2019 Dec 11;126(3):e10–e26. doi: 10.1161/CIRCRESAHA.119.315582

Figure 5: Desmin KD causes nuclear malformation in vivo.

Figure 5:

A, Sham (left) or desmin KD (right) rat left ventricular sections stained with desmin (orange) and DAPI (blue). The extra-nuclear blue signal is background fluorescence in the DAPI channel. Green and red arrows point to desmin-positive and desmin-negative cardiomyocyte nuclei, respectively. Scale = 10 μm. Cardiomyocyte nuclei were distinguished from non-myocyte nuclei via cTNT counterstaining (not shown). B, Zoomed-in images of representative desmin-positive and desmin-negative nuclei in tissue. Scale = 1 μm. C, Nuclear length (left), roundness (middle) and nuclear grading (right) of nuclei in tissue. *= compared to sham, #= compared to des+ cardiomyocytes. Sham: N=6 hearts, n= 188 nuclei. Desmin KD (des+): N=4, n= 75. Desmin KD (des-): N= 4, n= 100. Statistical significance determined via one-way ANOVA with post-hoc Bonferroni correction for length and roundness, and via Chi-squared test for blinded scoring. For statistical significance, * = p<0.05, ** = p<0.01, *** = p<0.001; # = p<0.05, ## = p<0.01, ### = p<0.001.