Table 1.

Preparation of riboprobe DIG labeling reaction

Reagent	Volume	Control #1	Control #2	Template (1×) Sense	Template (1×) Antisense	Template (2×) Antisense	Template (5×) Antisense
10× NTP mixture	2 μL	2 μL	2 μL	2 μL	2 μL	4 μL	10 μL
10× Transcription buffer	2 μL	2 μL	2 μL	2 μL	2 μL	4 μL	10 μL
Protector RNase A Inhibitor (20 units/μL)	1 μL	1 μL	1 μL	1 μL	1 μL	2 μL	5 μL
SP6 RNA polymerase (20 units/μL), OR	2 μL	-	2 μL	-	-	-	-
T7RNA polymerase (20 units/μL), OR	2 μL	2 μL	-	2 μL	-	-	-
T3 RNA polymerase (20 units/μL)	2 μL	-	-	-	2 μL	4 μL	10 μL
Total reaction volume	20 μL	20 μL	20 μL	20 μL	20 μL	40 μL	100 μL