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. Author manuscript; available in PMC: 2020 Feb 19.
Published in final edited form as: Methods Mol Biol. 2019;1950:107–122. doi: 10.1007/978-1-4939-9139-6_6

Table 3.

Preparation of RNA denaturation reaction

RNA 5 μL
5× MOPS Gel running buffer 2 μL
12.3 M Formaldehyde 3.5 μL
100% Formamide, deionized 10 μL
0.4 mg/mL ethidium bromide 1 μL
Total reaction volume 21.5 μL