. Author manuscript; available in PMC: 2020 Feb 19.

Published in final edited form as: Methods Mol Biol. 2019;1950:107–122. doi: 10.1007/978-1-4939-9139-6_6

Table 4.

Preparation of hybridization solution

Reagent	Amount (μL)
digoxigenin (DIG)-labeled riboprobe, 4 μg	X
Nuclease-free water	440-X
Tris-EDTA-NaCl buffer	360
100% deionized Formamide	2500
40% Dextran sulfate	1200
10% Blocking solution	500
Total	5000