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. Author manuscript; available in PMC: 2020 Feb 19.
Published in final edited form as: Methods Mol Biol. 2019;1950:107–122. doi: 10.1007/978-1-4939-9139-6_6

Table 5.

Preparation of antibody dilution buffer

Stock solutions Vol. stock solution Final concentration (antibody solution)
dd water 6.7/10.05/13.4/16.75 mL
5× Maleic acid buffer 2/3/4/5 mL 1× Maleate
10% Blocking solution 1.0/1.5/2.0/2.5 mL 1% Blocking solution
10% Triton X-100 0.3/0.45/0.6/0.75 mL 0.3% Triton X-100
Anti-Digoxigenin AP (ADD LAST) 4/6/8/10 μL 1:2500 dilution
Total volume 10/15/20/25 mL