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A) Sequence similarity network (SSN) of FAD-dependent monooxygenases (Pfam01494) using a sequence alignment score of 110. B) Results of expression, activity with model substrate S9 or S14**, site- and stereoselectivity of enzymes chosen from the SSN in panel A. *FDMO4 demonstrated <10% conversion by UPLC with substrate S9. C) Selected clusters from a more stringent SSN generated with an alignment score of 150 and corresponding analysis of the multisequence alignment of each cluster.
