Table 6.

Supplementation with 30 mg/kg BW/day XN or its derivatives DXN and TXN alters expression of genes involved in hepatic bile acid metabolism^d

Bile Acid Metabolism	CON	XN	DXN	TXN	SEM	XN vs CON	DXN vs CON	TXN vs CON
Gene	ΔCT					P-values (Comparisons)
Bile Acid Synthesis
Cyp7a1	Ref.	1.15	1.64	2.16	0.77	0.69	0.16	0.04
Cyp27a1	Ref.	1.21	−1.08	−1.15	0.87	0.33	0.68	0.49
Cyp8b1	Ref.	1.29	1.16	1.22	0.86	0.21	0.47	0.34
Bile Acid Secretion
Fxr	Ref.	−1.07	1.28	1.27	0.86	0.76	0.22	0.25
Shp	Ref.	1.91	3.62	2.98	0.74	0.12	0.003	0.01
Bsep	Ref.	1.03	−1.56	−1.84	0.86	0.91	0.04	0.008

^dAll the threshold cycle (Ct) numbers were normalized to a reference gene, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, Ywhaz, which others and we determined to be relatively stable in most mouse tissues (data not shown)^[17]. The fold-change compared to the control (HFD) was calculated using the 2^−ΔΔCt method. Ref: HFD control mice were used as the reference.