Figure 3. Preferential expression of EDS5-GFP in the epidermis of Arabidopsis. (A) flg22-induced expression of EDS5 in wild type and transgenic Arabidopsis plants expressing EDS5-GFP from the EDS5 promoter. Plants were treated with 1 μM flg22 for 2 and 8 h. ACT2 is shown as a control. (B) Overexpression of EDS5 in 35S:EDS5-GFP transformants. (C–G) Localization of the EDS5–GFP in epidermis cells (C and D), mesophyll cells (E), the inflorescence stem (F) and guard cells (G) of EDS5_prom:EDS5-GFP transformants. (H–K) Localization of the EDS5-GFP in epidermis cells (H), mesophyll cells (I), the inflorescence stem (J) and guard cells (K) of 35S:EDS5-GFP transformants. Scale bar, 20 µm.