表 1. 引物序列.
基因 | 编号 | 引物序列 |
ALP | NM_007431 | F:5′-tgcctacttgtgtggcgtgaa-3′ |
R:5′-tcacccgagtggtagtcacaatg-3′ | ||
OCN | NM_007541 | F:5′-agcagcttggcccagaccta-3′ |
R:5′-tagcgccggagtctgttcactac-3′ | ||
ColⅠ | NM_007742 | F:5′-atgccgcgacctcaagatg-3′ |
R:5′-tgaggcacagacggctgagta-3′ | ||
Runx2 | NM_009820 | F:5′-cactggcggtgcaacaaga-3′ |
R:5′-tttcataacagcggaggcatttc-3′ | ||
β-actin | NM_007393 | F:5′-aggagcaatgatcttgatctt-3′ |
R:5′-tgccaacacagtgctgtct-3′ |