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. 2020 Jan 16;5(1):e121781. doi: 10.1172/jci.insight.121781

Figure 6. miR-543 leads to decreased protein acetylation.

Figure 6

(A) Percentage of overall 5-mC methylation levels in K562 cells stably transduced with empty vector (control) or p–miR-543 expression vector. Percentage of H19 differentially methylated region (DMR) 5-mC methylation levels in K562 cells stably transduced with empty vector (control) or p–miR-543 expression vector after being transiently transfected with TET1 or TET2 siRNA. (B) Percentage of overall histone 3 (H3) acetylation levels in K562 cells stably transduced with empty vector (control) or p–miR-543 expression vector. Gene enrichment analysis of genes related to H3 acetylation in K562 cells overexpressing miR-543. (C) Representative Western blot analysis of acetylated STAT3 protein in K562 parental cells and stably transduced with empty vector (control) or p–miR-543 expression vector. β-Actin protein levels were used as normalizer. (D) Gene enrichment analysis of genes upregulated by STAT3 pathway activation in K562 cells overexpressing miR-543. Two-tailed t test or Mann-Whitney-Wilcoxon nonparametric test was used to assess statistical significance for A and B. All experiments were repeated independently 3 times, and representative blots are shown.