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. 2020 Jan 16;5(1):e133282. doi: 10.1172/jci.insight.133282

Figure 4. Ouabain decreases autotic cell death and improves outcome in renal IRI.

Figure 4

(A and B) Representative micrographs (A) and quantitation (B) of autotic pericytes in the kidneys from mice that were subjected to sham operation or renal IRI after peritoneal administration of either vehicle or ouabain (0.25 mg/kg). In B, bars represent mean ± SEM (n = 5–8 mice per group, more than 50 cells analyzed per mouse). (C and D) Representative H&E images (C) and histological score (D) of kidneys from mice that were subjected to sham operation or renal IRI after peritoneal administration of either vehicle or ouabain (0.25 mg/kg). In D, bars represent mean ± SEM. (n = 5–8 mice per group, 10 randomly selected fields analyzed per mouse). (E and F) Assessment of renal damage as measured by blood urea nitrogen (BUN) (E) and creatinine (F) levels in plasma from mice that were subjected to sham operation or renal IRI after peritoneal administration of either vehicle or ouabain (0.25 mg/kg). Bars represent mean ± SD (n = 5–8 mice per group). Scale bars: 1 μm (A), 50 μm (C). **P < 0.01; ***P < 0.001; 2-way ANOVA test.