KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| NG2 | Abcam | Cat#ab83508; RRID:AB_2087616. ab50009; RRID:AB_881569. ab83178; RRID:AB_10672215 |
| PDGFRα | Santa Cruz | Cat#sc-338; RRID:AB_631064. sc-398206; RRID:N/A |
| αSMA-Cy3 | Sigma | Cat#C6198; RRID:AB_476856 |
| GFP-FITC | Abcam | Cat#ab6662; RRID:AB_305635 |
| PDGFRα-APC | eBioscience | Cat#17-1401-81; RRID:AB_529482 |
| NG2-APC | R&D | Cat#FAB2585A; RRID:AB_622049 |
| CD31-APC | BioLegend | Cat#102409; RRID:AB_312904 |
| Sca1-PE | Becton Dickinson | Cat#553108; RRID:AB_394629 |
| NG2-PE | BD PharMingen | Cat# 562415; RRID:AB_11154031 |
| CD45-APC-Cy7 | Becton Dickinson | Cat#557659; RRID:AB_396774 |
| CD44-PE | eBioscience | Cat#12-0441-82; RRID:AB_465664 |
| CD31-PE | Abcam | Cat#ab25644; RRID:AB_470721 |
| Collagen1 | Abcam | Cat#ab34710; RRID:AB_731684 |
| PE rat IgG2a, kappa | BD-Bioscience | Cat#553930; RRID:AB_479719 |
| APC rat IgG2a kappa | eBioscience | Cat#17-4321-81; RRID:AB_470181 |
| PE mouse IgG2a, kappa | BD-Bioscience | Cat#555574; RRID:AB_395953 |
| PE rat IgG2A | R&D systems | Cat# IC006P; RRID:AB_357256 |
| PE Armenian hamster IgG | Biolegend | Cat#400907; RRID:AB_326593 |
| PE rat IgG1, kappa | Biolegend | Cat#400407; RRID:AB_326513 |
| PE rat IgG2a, kappa | Biolegend | Cat#400507; RRID:N/A |
| PE rat IgG2a, kappa | eBioscience | Cat#12-4321-80; RRID:AB_1834380 |
| PE rat IgG2b, kappa | eBioscience | Cat#12-4031-82; RRID:AB_470042 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Evans blue dye | Sigma | Cat#E2129 |
| Paraformaldehyde | EMS | Cat#15710 |
| OCT | Tissue-Tek | Cat#4583 |
| Vascular endothelial growth factor A, VEGF-A | Peprotech | Cat#450–32 |
| Platelet derived-growth factor AB, PDGF-AB | R&D | Cat#1115-AB-010 |
| Biotinylated isolectin B4 | Sigma | Cat#L2140 |
| Tamoxifen (> 99%) | Sigma | Cat#T-5648 |
| Diphteria Toxin, DTX | Sigma | Cat#D0564 |
| Growth Factor Reduced BD Matrigel Membrane | BD Bioscience | Cat#356230 |
| DMEM, High glucose/L-glutamine | Life technologies | Cat#11965–096 |
| EGM-2 SingleQuot Kit Suppl. & Growth Factors | Lonza | Cat#CC4176 |
| EBM2 basal media | Lonza | Cat#CC3156 |
| Fetal Bovine Serum | Life Technologies | Cat#16000044 |
| Strepdavidin conjugated HRP | Abcam | Cat#ab64269 |
| DAB | Sigma | Cat#D3939–1SET |
| Critical Commercial Assays | ||
| RNAeasy mini kit | QIAGEN | Cat#74104 |
| Direct Red 80 | Sigma | Cat# 365548 |
| Ovation RNA sequence V2 | NuGen Technologies | Cat#7102–32 |
| Senescence Cells Histochemical Staining Kit | Sigma | Cat#CS0030 |
| Deposited Data | ||
| RNaseq data | This paper | GEO: GSE101930 |
| Experimental Models: Cell Lines | ||
| HUVEC | Lonza | Cat#C2517A |
| PDGFRαH2B-eGFP cells (from PdgfrαH2B-eGfp mice) | This paper | N/A |
| Experimental Models: Organisms/Strains | ||
| Mouse: Nude, Strain NU/J, inbred for 100 generations | Jackson Laboratories | Cat#002019; RRID:IMSR_JAX:002019 |
| Mouse: PdgfrαH2B-eGfp, Strain B6.129S4-Pdgfratm11(EGFP)Sor/J | Jackson Laboratories | Cat#007669; RRID:IMSR_JAX:007669 |
| Mouse: IDTR, Strain C57BL/6-Gt(ROSA)26Sortm1 (HBEGF)Awai/J | Jackson Laboratories | Cat#007900; RRID:IMSR_JAX:007900 |
| Mouse: MCM, Strain PdgfrαMerCreMer | Riken | Accession# CDB0674K, http://www2.clst.riken.jp/arg/mutant%20mice%20list.html; RRID:N/A |
| Mouse: Brainbow 2.1, Strain B6.129P2-Gt(ROSA) 26Sortm1(CAG-Brainbow2.1)Cle/J. | Jackson Laboratories | Cat#017492; RRID:IMSR_JAX:017492; |
| Mouse: R26R-eYfp, Strain B6.129X1-Gt (ROSA)26Sortm1(eYfp)Cos/J | Jackson Laboratories | Cat#006148; RRID:IMSR_JAX:006148 |
| Sequence-Based Reagents | ||
| Ovation RNA sequence V2 | NuGen Technologies | Cat#7102–32 |
| Software and Algorithms | ||
| GraphPad Prism V.6® | GraphPad Software, La Jolla, USA | https://www.graphpad.com/scientific-software/prism/; RRID:SCR_002798 |
| ImageJ | NIH | https://imagej.nih.gov/ij/; RRID:SCR_003070 |
| Fiji | NIH | http://fiji.sc; RRID:SCR_002285 |
| Cytobank Community | Cytobank Inc. | https://www.cytobank.org/index.html; RRID:SCR_014043 |
| Imaris V8.4 | Bitplane | https://imaris.oxinst.com/packages; RRID:SCR_007370 |