Figure 4. Influence of HA on PrP^Sc: (A) interactions between HA and PrP^CWD affect PrP^CWD recovery and molecular weight. Identical amounts of 10% brain homogenate (BH^CWD) were incubated with water (control) and HA (1g/L, 2.5 g/L and 25 g/L) overnight at room temperature. Samples were analyzed for presence of PK-resistant PrP^CWD by western blot with Bar224 antibody. (B) HA affect hamster scrapie prion (PrP^HY) binding to Mte. 10% brain homogenate (BH^HY) was incubated with Mte ± HA (1 g/L and 25 g/L) overnight at room temperature. Samples were fractionated through a sucrose cushion to separate bound PrP^HY from unbound; residual (r; adsorbed on the Eppendorf tube PrP^HY), pellet (p; bound PrP^HY) and supernatant (s; unbound PrP^HY) were analyzed for presence of PK-resistant PrP^HY by western blot with 3F4 antibody.