Figure 1. Modulation of fibril formation of rHuPrP90–231 by the M129V mutant. (A) Lag phase and (C) growth rate of 129M spontaneous (gray), seeded with 129M (blue) and seeded with 129V (red). (B) Lag phase and (D) Growth rate of 129V spontaneous (gray), seeded with 129V (blue) and seeded with 129M (red). All data represent ThT fibril formation kinetics in vitro (n = 6), in the native condition conversion assay described in Almstedt et al., 2009.⁶⁰