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Methionine (1a) can be reacted with ATP analogues (2-8) by ChMAT A) or hMAT2a I117A B). The resulting AdoMet analogues 9a-15a can be used as cofactors by a suitable methyltransferase as shown here for Ecm1. Conditions: 6 mM 1a, 2.5 mM 2-8, 1 mol% MAT (rel. to 2-8), 300 μM 16, 10 mol% Ecm1 (rel. to 16). Reaction mixture was incubated at 23 °C and analyzed by HPLC at time points indicated.
