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. Author manuscript; available in PMC: 2021 Feb 18.
Published in final edited form as: Biochemistry. 2020 Jan 21;59(6):802–817. doi: 10.1021/acs.biochem.9b01012

Figure 2. MRP126 selectively depletes Zn from bacterial growth medium using its His3Asp sites.

Figure 2.

Depletion of (A) Mn, (B) Fe, (C) Co, (D) Ni, (E) Cu, and (F) Zn. Tris:TSB medium was incubated with 8 μM of MRP126, ΔTail, ΔHis3Asp, D36H, or 10 μM hCP for 20 h at 30 °C. Following spin filtration to remove proteins > 10 kDa, the metal content of the filtrate was determined by ICP-MS (mean ± SEM, n ≥ 4). The medium contained no supplement (white bars), a 3-mM BME supplement (light gray bars), a 2-mM Ca(II) supplement (medium gray bars), or both 2-mM Ca(II) and 3-mM BME supplements (dark gray bars). Raw ICP-MS data are provided in Table S5.