Skip to main content
. Author manuscript; available in PMC: 2020 Jul 20.
Published in final edited form as: Nat Genet. 2020 Jan 20;52(2):138–145. doi: 10.1038/s41588-019-0568-7

Extended Data Fig. 7:

Extended Data Fig. 7:

Effects of high-level LIN28B expression in adult erythroid cells

a, BCL11A mRNA levels (normalized to GAPDH expression), upon high-level LIN28B expression (GFP-high) in adult erythroid cells, assessed on differentiation day 7 (n = 3; 3 biologically independent experiments). Mean is plotted and error bars show s.d. Two-sided Student t-test used. **P < 0.01. b, Relative γ-globin expression as a percentage of total globins (γ- and β-globins), upon high-level LIN28B expression (GFP-high) in adult erythroid cells, on differentiation day 15 (n=3; 3 independent experiments). Error bars show s.d. ***P < 0.001. c, Representative flow cytometry plots showing CD71 and CD235a surface expression in control (left) and physiological level LIN28B expressing (right) adult erythroid cells at day 7 of differentiation. Data represents mean ± s.d., n = 3; 3 biologically independent experiments.