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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: J Hepatol. 2019 Oct 10;72(3):391–400. doi: 10.1016/j.jhep.2019.09.029

Fig. 2. Effects of Candidalysin on ethanol-induced liver disease.

Fig. 2.

C57BL/6 mice were fed an oral isocaloric (control) diet (1–2 technical replicates) or chronic-plus-binge ethanol diet (3–4 technical replicates), and gavaged with vehicle (PBS), wild type C. albicans (Wild type), ECE1 deleted C. albicans (ece1Δ/Δ), only Ece1-III62–93 deleted C. albicans (ece1Δ/Δ + ECE1Δ184–279) or ECE1 re-integrant C. albicans (ece1Δ/Δ + ECE1) with an amount of 108 colony forming units (CFUs) every third day. (A) Serum levels of alanine aminotransferase (ALT). (B) Hepatic triglycerides levels. (C) Representative images of Oil Red O stained liver tissue. (D) Hepatic levels of Il1b mRNA. (E) Hepatic levels of Cxcl1 mRNA. (F) Hepatic levels of Cxcl2 mRNA. (G) Serum levels of ethanol in mice fed chronic-plus-binge ethanol diet. (H) Hepatic levels of Adh1 mRNA. (I) Hepatic levels of Cyp2e1 mRNA. (Control diet: PBS, n=8; Wild type, n=5; ece1Δ/Δ, n=5; ece1Δ/Δ + ECE1Δ184–279, n=5; ece1Δ/Δ + ECE1, n=5; Ethanol diet: PBS, n=14; Wild type, n=14; ece1Δ/Δ, n=14; ece1Δ/Δ + ECE1Δ184–279, n=14; ece1Δ/Δ + ECE1, n=12). Scale bars = 100 μm. Images were taken at ×100 magnification. Results are expressed as mean ± s.e (A, B, D - I). P values were determined by one-way ANOVA with Tukey’s post-hoc test (A, B, D - I). *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.

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